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CaMKII delta-Specific Polyclonal antibody
CaMKII delta-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20667-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, mouse heart tissue, human skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20667-1-AP targets CaMKII delta-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | calcium/calmodulin-dependent protein kinase II delta |
| Calculated Molecular Weight | 59 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_001221 |
| Gene Symbol | CAMK2D |
| Gene ID (NCBI) | 817 |
| RRID | AB_10695498 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| IHC protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| IP protocol for CaMKII delta-Specific antibody 20667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p. | ||
Cell Death Dis miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2. | ||
Reprod Biol Endocrinol In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein | ||
J Pain Res Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway. | ||
Cell Mol Life Sci JOSD2 mediates isoprenaline-induced heart failure by deubiquitinating CaMKIIδ in cardiomyocytes |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jonathan (Verified Customer) (11-11-2019) | Works well for WB (1:1000) and IF (1:200)
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