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CaMKII gamma-Specific Polyclonal antibody

CaMKII gamma-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55140-1-AP

Synonyms

CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CaMKII gamma-Specific Polyclonal antibody (55140-1-AP)

    WB analysis of mouse brain using 55140-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CaMKII gamma-Specific Polyclonal antibody (55140-1-AP)

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CaMKII gamma-Specific Polyclonal antibody (55140-1-AP)

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

55140-1-AP targets CaMKII gamma-Specific in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium/calmodulin-dependent protein kinase II gamma
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 45-65 kDa
GenBank Accession NumberNM_001222
Gene Symbol CAMK2G
Gene ID (NCBI) 818
RRIDAB_10858395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII gamma, also named as CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CaMK2 is a Ca2+-signaling intermediate that contains α, β, γ and δ subunits. The antibody is specific to CAMK2G.

Protocols

Product Specific Protocols
WB protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
IHC protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
View Article
humanWB

Biomed Pharmacother

Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.

Authors - Qingfeng Yu
View Article