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CARMIL Polyclonal antibody

CARMIL Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27133-1-AP

Synonyms

CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CARMIL Polyclonal antibody (27133-1-AP)

    WB analysis of MCF-7 using 27133-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CARMIL Polyclonal antibody (27133-1-AP)

    WB analysis using 27133-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CARMIL Polyclonal antibody (27133-1-AP)

    WB analysis of HeLa using 27133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CARMIL Polyclonal antibody (27133-1-AP)

    IP experiment of MCF-7 using 27133-1-AP

    IP result of anti-CARMIL (IP:27133-1-AP, 4ug; Detection:27133-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using CARMIL Polyclonal antibody (27133-1-AP)

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CARMIL Polyclonal antibody (27133-1-AP)

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27133-1-AP is CARMIL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARMIL fusion protein Ag25876
Full Name leucine rich repeat containing 16A
Observed Molecular Weight 152 kDa
GenBank Accession NumberBC140770
Gene Symbol LRRC16A
Gene ID (NCBI) 55604
RRIDAB_2880768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CARMIL antibody 27133-1-APDownload protocol
IHC protocol for CARMIL antibody 27133-1-APDownload protocol
IP protocol for CARMIL antibody 27133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols