PE Anti-Mouse CD45.2 (104)

CD45.2 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

104

Cat no : PE-65072

Synonyms

B220, CD45.2, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65072 targets CD45.2 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen B10.S mouse thymocytes and splenocytes
Full Name protein tyrosine phosphatase, receptor type, C
GenBank Accession NumberBC028512
Gene Symbol Ptprc
Gene ID (NCBI) 19264
RRIDAB_2883869
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

Protocols

Product Specific Protocols
FC protocol for PE CD45.2 antibody PE-65072Download protocol
Standard Protocols
Click here to view our Standard Protocols