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Anti-Human CD9 (MM2/57)

CD9 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

MM2/57

Cat no : 65070-1-Ig

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using Anti-Human CD9 (MM2/57) (65070-1-Ig)

    FC experiment of human peripheral blood platelets using 65070-1-Ig

    1X10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD9 (65070-1-Ig, Clone: MM2/57) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug mouse IgG2b isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65070-1-Ig targets CD9 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD9 molecule
Calculated Molecular Weight 228 aa, 25 kDa
GenBank Accession NumberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
RRIDAB_2918376
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

Protocols

Product Specific Protocols
FC protocol for CD9 antibody 65070-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols