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CDC5L Polyclonal antibody

CDC5L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12974-1-AP

Synonyms

Cdc5 like protein, CDC5L, CEF1, hCDC5, KIAA0432, PCDC5RP, Pombe cdc5 related protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of human brain using 12974-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of HEK-293 using 12974-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of HepG2 using 12974-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of HepG2 using 12974-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of mouse brain using 12974-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDC5L Polyclonal antibody (12974-1-AP)

    WB analysis of HEK-293 using 12974-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CDC5L Polyclonal antibody (12974-1-AP)

    IP experiment of mouse brain using 12974-1-AP

    IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CDC5L Polyclonal antibody (12974-1-AP)

    IHC staining of mouse testis using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CDC5L Polyclonal antibody (12974-1-AP)

    IHC staining of mouse testis using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDC5L Polyclonal antibody (12974-1-AP)

    IHC staining of mouse brain using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDC5L Polyclonal antibody (12974-1-AP)

    IHC staining of mouse brain using 12974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC5L Polyclonal antibody (12974-1-AP)

    IF Staining of HepG2 using 12974-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12974-1-AP (CDC5L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12974-1-AP targets CDC5L in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC5L fusion protein Ag3578
Full Name CDC5 cell division cycle 5-like (S. pombe)
Calculated Molecular Weight 802 aa, 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC001568
Gene Symbol CDC5L
Gene ID (NCBI) 988
RRIDAB_2078510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDC5L antibody 12974-1-APDownload protocol
IHC protocol for CDC5L antibody 12974-1-APDownload protocol
IF protocol for CDC5L antibody 12974-1-APDownload protocol
IP protocol for CDC5L antibody 12974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
View Article
WB

J Biol Chem

O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.

Authors - Li Liu
View Article
humanWB

Cancer Med

Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.

Authors - Chunmei Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wael (Verified Customer) (01-19-2023)

CDC5L detected in both nuclear and cytoplasmic fractions of Hek293 lysates. specific band between 100 and 130kd.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hek293
CDC5L Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:12974-1-AP)