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Cadherin-17 Monoclonal antibody

Cadherin-17 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B10A9

Cat no : 60351-1-Ig

Synonyms

Cadherin-17, CDH16, CDH17, HPT 1, HPT1, LI cadherin, Liver intestine cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of pig colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of pig colon using 60351-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    WB analysis of human colon using 60351-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human stomach cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human colon cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IHC staining of human pancreas cancer using 60351-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60351-1-Ig (Cadherin-17 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Cadherin-17 Monoclonal antibody (60351-1-Ig)

    IF Staining of human colon cancer using 60351-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Cadherin-17 antibody (60351-1-Ig, Clone: 3B10A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman colon tissue, pig colon tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human pancreas cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:16000-1:64000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60351-1-Ig targets Cadherin-17 in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cadherin-17 fusion protein Ag19488
Full Name cadherin 17, LI cadherin (liver-intestine)
Calculated Molecular Weight 832 aa, 92 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC113464
Gene Symbol CDH17
Gene ID (NCBI) 1015
RRIDAB_2881460
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
IHC protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
IF protocol for Cadherin-17 antibody 60351-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biochim Biophys Acta Gen Subj

Gastric cancer patient-derived organoids model for the therapeutic drug screening

Authors - Jiao Xu
View Article