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CGNL1 Polyclonal antibody

CGNL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18031-1-AP

Synonyms

CGNL1, cingulin like 1, Cingulin like protein 1, JACOP, KIAA1749, Paracingulin

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CGNL1 Polyclonal antibody (18031-1-AP)

    WB analysis of NIH/3T3 using 18031-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18031-1-AP (CGNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CGNL1 Polyclonal antibody (18031-1-AP)

    WB analysis of HEK-293 using 18031-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18031-1-AP (CGNL1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CGNL1 Polyclonal antibody (18031-1-AP)

    IHC staining of human liver cancer using 18031-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18031-1-AP (CGNL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CGNL1 Polyclonal antibody (18031-1-AP)

    IHC staining of human liver cancer using 18031-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18031-1-AP (CGNL1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18031-1-AP is CGNL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CGNL1 fusion protein Ag12598
Full Name cingulin-like 1
Calculated Molecular Weight 1302 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC093827
Gene Symbol CGNL1
Gene ID (NCBI) 84952
RRIDAB_10644446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CGNL1 antibody 18031-1-APDownload protocol
IHC protocol for CGNL1 antibody 18031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,IHC

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu
View Article
humanWB

Sci Rep

Single-cell and bulk RNA sequencing reveals Anoikis related genes to guide prognosis and immunotherapy in osteosarcoma

Authors - Cheng Zhong
View Article