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  • Featured Product
  • KD/KO Validated

CNOT3 Polyclonal antibody

CNOT3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11135-1-AP

Synonyms

CCR4 associated factor 3, CNOT3, KIAA0691, LENG2, NOT3, NOT3H

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of Raji using 11135-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of human brain using 11135-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of K-562 using 11135-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of HT-1080 using 11135-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of mouse brain using 11135-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CNOT3 Polyclonal antibody (11135-1-AP)

    WB analysis of HEK-293 using 11135-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11135-1-AP (CNOT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CNOT3 Polyclonal antibody (11135-1-AP)

    IP experiment of HeLa using 11135-1-AP

    IP result of anti-CNOT3 (IP:11135-1-AP, 4ug; Detection:11135-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNOT3 Polyclonal antibody (11135-1-AP)

    IF Staining of HeLa using 11135-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CNOT3 antibody (11135-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRaji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11135-1-AP targets CNOT3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT3 fusion protein Ag1587
Full Name CCR4-NOT transcription complex, subunit 3
Calculated Molecular Weight 120 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC016474
Gene Symbol CNOT3
Gene ID (NCBI) 4849
RRIDAB_2229682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].

Protocols

Product Specific Protocols
WB protocol for CNOT3 antibody 11135-1-APDownload protocol
IF protocol for CNOT3 antibody 11135-1-APDownload protocol
IP protocol for CNOT3 antibody 11135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.

Authors - G Gregory Neely
  • KO Validated
View Article
humanWB

Nucleic Acids Res

Alternative splicing of CNOT7 diversifies CCR4-NOT functions.

Authors - Clément Chapat
View Article
humanWB

RNA

An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.

Authors - Ewan K S McRae
View Article
humanWB

Apoptosis

PMID: 31177396

Authors - Lin Jing
  • KD Validated
View Article
mouseWB,IF

bioRxiv

Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance

Authors - Qing Chen
View Article
humanWB

Nat Cancer

ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.

Authors - Tong Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hannah (Verified Customer) (02-06-2019)

Works well and specifically for WB on human fibroblast lysates.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/5000 overnight
  • Cell Tissue Type: human fibroblast (NHDF)
CNOT3 Antibody Western Blot, validation (1/5000 overnight dilution) in human fibroblast (NHDF) (Cat no:11135-1-AP)