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  • Featured Product
  • KD/KO Validated

CNOT6 Polyclonal antibody

CNOT6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17935-1-AP

Synonyms

CCR4, CNOT6, Cytoplasmic deadenylase, KIAA1194

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CNOT6 Polyclonal antibody (17935-1-AP)

    WB analysis of Jurkat using 17935-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17935-1-AP (CNOT6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17935-1-AP targets CNOT6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT6 fusion protein Ag11506
Full Name CCR4-NOT transcription complex, subunit 6
Calculated Molecular Weight557 aa, 63.3 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC027476
Gene Symbol CNOT6
Gene ID (NCBI) 57472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells. This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (PMID: 12882519). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (PMID: 19558367). In addition, the subunit composition of this complex has been shown to vary among different tissues (PMID: 21741365). The various CNOT proteins display different functions, with CNOT6, CNOT6L, CNOT7, and CNOT8 exhibiting 3'-5' RNAse activity (PMID: 11889047). Research studies indicate that the CCR4-NOT deadenylase subunits CNOT6 and CNOT6L help mediate cell survival and play a role in cell proliferation, P-body formation, and regulation of gene expression (PMID: 21233283). Additional research evidence suggests that CNOT6 can act as a nuclear hormone receptor coactivator that associates with ZNF335 (NIF-1) to regulate expression from specific RARα regulated genes (PMID: 18180299).

Protocols

Product Specific Protocols
WB protocol for CNOT6 antibody 17935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis.

Authors - Lan-Tao Gou
  • KD Validated
View Article