CNTFR Polyclonal antibody

CNTFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10796-1-AP

Synonyms

CNTF receptor subunit alpha, CNTFR, CNTFR alpha



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, SH-SY5Y cells, rat brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10796-1-AP targets CNTFR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTFR fusion protein Ag1189
Full Name ciliary neurotrophic factor receptor
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC001492
Gene Symbol CNTFR
Gene ID (NCBI) 1271
RRIDAB_2083194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

Protocols

Product Specific Protocols
WB protocol for CNTFR antibody 10796-1-APDownload protocol
IHC protocol for CNTFR antibody 10796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Pharmacol

Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Authors - Bin Yu