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CNTROB Monoclonal antibody

CNTROB Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D12A4

Cat no : 67061-1-Ig

Synonyms

Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis using 67061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis of pig brain using 67061-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CNTROB Monoclonal antibody (67061-1-Ig)

    WB analysis of rat brain using 67061-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CNTROB Monoclonal antibody (67061-1-Ig)

    IF Staining of hTERT-RPE1 using 67061-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CNTROB antibody (67061-1-Ig, Clone: 3D12A4 ) at dilution of 1:350 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

Tested Applications

Positive WB detected inA549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:175-1:700
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67061-1-Ig targets CNTROB in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNTROB fusion protein Ag25447
Full Name centrobin, centrosomal BRCA2 interacting protein
Calculated Molecular Weight 903 aa, 101 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC021134
Gene Symbol CNTROB
Gene ID (NCBI) 116840
RRIDAB_2882371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CNTROB antibody 67061-1-IgDownload protocol
IF protocol for CNTROB antibody 67061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols