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  • Featured Product
  • KD/KO Validated

CPSF1 Polyclonal antibody

CPSF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11031-1-AP

Synonyms

CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of Jurkat using 11031-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis using 11031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of HeLa using 11031-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of human brain using 11031-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, human brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11031-1-AP targets CPSF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPSF1 fusion protein Ag1489
Full Name cleavage and polyadenylation specific factor 1, 160kDa
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC017232
Gene Symbol CPSF1
Gene ID (NCBI) 29894
RRIDAB_2245276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

Protocols

Product Specific Protocols
WB protocol for CPSF1 antibody 11031-1-APDownload protocol
IHC protocol for CPSF1 antibody 11031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
View Article
humanWB

J Biosci

Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian cancer.

Authors - Beiguang Zhang
  • KD Validated
View Article