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  • Featured Product
  • KD/KO Validated

CYP4F11 Polyclonal antibody

CYP4F11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12286-1-AP

Synonyms

CYP4F11, CYPIVF11, Cytochrome P450 4F11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis using 12286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis using 12286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis of human liver using 12286-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis of MCF-7 using 12286-1-AP

    WB result of CYP4F11 antibody (12286-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F11 transfected MCF-7 cells.

  • Western Blot (WB) analysis of human liver tissue using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis of human liver using 12286-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CYP4F11 Polyclonal antibody (12286-1-AP)

    WB analysis of human kidney using 12286-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP4F11 Polyclonal antibody (12286-1-AP)

    IHC staining of human liver cancer using 12286-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP4F11 Polyclonal antibody (12286-1-AP)

    IHC staining of human liver cancer using 12286-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CYP4F11 Polyclonal antibody (12286-1-AP)

    IF Staining of A549 using 12286-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CYP4F11 antibody (12286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12286-1-AP targets CYP4F11 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP4F11 fusion protein Ag2906
Full Name cytochrome P450, family 4, subfamily F, polypeptide 11
Calculated Molecular Weight 524 aa, 60 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC016853
Gene Symbol CYP4F11
Gene ID (NCBI) 57834
RRIDAB_2088407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)

Protocols

Product Specific Protocols
WB protocol for CYP4F11 antibody 12286-1-APDownload protocol
IHC protocol for CYP4F11 antibody 12286-1-APDownload protocol
IF protocol for CYP4F11 antibody 12286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Metab Dispos

Gene regulation of CYP4F11 in human keratinocyte HaCaT cells.

Authors - Wang Ying Y
View Article
humanChIP

Drug Metab Dispos

Regulation of cytochrome P450 4F11 by nuclear transcription factor-κB.

Authors - Bell Jordan C JC
View Article