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DAB2IP Polyclonal antibody

DAB2IP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23582-1-AP

Synonyms

AF9Q34, AIP1, ASK interacting protein 1, DAB2 interacting protein, DAB2 interaction protein, DAB2IP, DIP1/2, KIAA1743

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAB2IP Polyclonal antibody (23582-1-AP)

    WB analysis using 23582-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23582-1-AP (DAB2IP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DAB2IP Polyclonal antibody (23582-1-AP)

    IP experiment of mouse brain using 23582-1-AP

    IP result of anti-DAB2IP (IP:23582-1-AP, 4ug; Detection:23582-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23582-1-AP targets DAB2IP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAB2IP fusion protein Ag20308
Full Name DAB2 interacting protein
Calculated Molecular Weight 1189 aa, 132 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC146762
Gene Symbol DAB2IP
Gene ID (NCBI) 153090
RRIDAB_2879300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DAB2IP antibody 23582-1-APDownload protocol
IP protocol for DAB2IP antibody 23582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF, IHC

Cancer Sci

DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells.

Authors - Haiou Li
View Article
humanWB,IHC

Biochim Biophys Acta Mol Basis Dis

DNMT3A facilitates colorectal cancer progression via regulating DAB2IP mediated MEK/ERK activation.

Authors - Yunjiao Zhou
View Article
humanWB,IHC,IF

Cell Death Dis

Soft fibrin matrix downregulates DAB2IP to promote Nanog-dependent growth of colon tumor-repopulating cells.

Authors - Meng Zhang
View Article