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DAD1 Polyclonal antibody

DAD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10531-1-AP

Synonyms

DAD 1, DAD1, defender against cell death 1, OST2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAD1 Polyclonal antibody (10531-1-AP)

    WB analysis using 10531-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10531-1-AP (DAD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using DAD1 Polyclonal antibody (10531-1-AP)

    IP experiment of HepG2 using 10531-1-AP

    IP result of anti-DAD1 (IP:10531-1-AP, 4ug; Detection:10531-1-AP 1:700) with HepG2 cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DAD1 Polyclonal antibody (10531-1-AP)

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DAD1 Polyclonal antibody (10531-1-AP)

    IHC staining of human liver cancer using 10531-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10531-1-AP (DAD1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DAD1 Polyclonal antibody (10531-1-AP)

    IF Staining of HepG2 using 10531-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10531-1-AP (DAD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10531-1-AP targets DAD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAD1 fusion protein Ag0814
Full Name defender against cell death 1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC007403
Gene Symbol DAD1
Gene ID (NCBI) 1603
RRIDAB_2089878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAD1 (defender against cell death 1) is a downstream target of the NFkB survival pathway and exhibits an antiapoptotic function. It is also named as OST2 (Oligosaccharyl transferase2) and catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Increased expression of DAD1 has been observed in various tumors.

Protocols

Product Specific Protocols
WB protocol for DAD1 antibody 10531-1-APDownload protocol
IHC protocol for DAD1 antibody 10531-1-APDownload protocol
IF protocol for DAD1 antibody 10531-1-APDownload protocol
IP protocol for DAD1 antibody 10531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols