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DDX28 Polyclonal antibody

DDX28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11617-1-AP

Synonyms

DDX28, MDDX28

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX28 Polyclonal antibody (11617-1-AP)

    WB analysis using 11617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11617-1-AP (DDX28 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DDX28 Polyclonal antibody (11617-1-AP)

    IHC staining of human prostate cancer using 11617-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11617-1-AP (DDX28 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293T cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11617-1-AP targets DDX28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX28 fusion protein Ag2194
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
Calculated Molecular Weight 540 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC024273
Gene Symbol DDX28
Gene ID (NCBI) 55794
RRIDAB_2092574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DDX28 antibody 11617-1-APDownload protocol
IHC protocol for DDX28 antibody 11617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.

Authors - Christopher Lowden
View Article