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DHPS Polyclonal antibody

DHPS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11184-1-AP

Synonyms

deoxyhypusine synthase, DHPS, DHS, DS, MIG13

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DHPS Polyclonal antibody (11184-1-AP)

    WB analysis of HeLa using 11184-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11184-1-AP (DHPS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using DHPS Polyclonal antibody (11184-1-AP)

    IP experiment of NIH/3T3 using 11184-1-AP

    IP result of anti-DHPS (IP:11184-1-AP, 4ug; Detection:11184-1-AP 1:300) with NIH/3T3 cells lysate 3200 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DHPS Polyclonal antibody (11184-1-AP)

    IF Staining of HeLa using 11184-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DHPS antibody (11184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11184-1-AP targets DHPS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHPS fusion protein Ag1646
Full Name deoxyhypusine synthase
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2877751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DHPS antibody 11184-1-APDownload protocol
IF protocol for DHPS antibody 11184-1-APDownload protocol
IP protocol for DHPS antibody 11184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

BMC Med

Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.

Authors - Takaki Hiwasa
View Article