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DNAJB8 Polyclonal antibody

DNAJB8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17071-1-AP

Synonyms

DJ6, DNAJB8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DNAJB8 Polyclonal antibody (17071-1-AP)

    WB analysis of HeLa using 17071-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAJB8 Polyclonal antibody (17071-1-AP)

    WB analysis of human brain using 17071-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17071-1-AP targets DNAJB8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB8 fusion protein Ag10899
Full Name DnaJ (Hsp40) homolog, subfamily B, member 8
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC029521
Gene Symbol DNAJB8
Gene ID (NCBI) 165721
RRIDAB_2094743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJB8 antibody 17071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
View Article
mouseWB,IF

STAR Protoc

Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.

Authors - Qianxing Zou
View Article