DNAJC2/MPP11 Polyclonal antibody
DNAJC2/MPP11 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11971-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, PC-3 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
11971-1-AP targets DNAJC2/MPP11 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJC2/MPP11 fusion protein Ag2594 |
Full Name | DnaJ (Hsp40) homolog, subfamily C, member 2 |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 66-75 kDa |
GenBank Accession Number | BC056682 |
Gene Symbol | DNAJC2 |
Gene ID (NCBI) | 27000 |
RRID | AB_10695893 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for DNAJC2/MPP11 antibody 11971-1-AP | Download protocol |
IHC protocol for DNAJC2/MPP11 antibody 11971-1-AP | Download protocol |
IF protocol for DNAJC2/MPP11 antibody 11971-1-AP | Download protocol |
IP protocol for DNAJC2/MPP11 antibody 11971-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO J Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |