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  • Featured Product
  • KD/KO Validated

DNAJC21 Polyclonal antibody

DNAJC21 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23411-1-AP

Synonyms

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DNAJC21 Polyclonal antibody (23411-1-AP)

    WB analysis of HEK-293 using 23411-1-AP

    WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.

  • Western Blot (WB) analysis of various lysates using DNAJC21 Polyclonal antibody (23411-1-AP)

    WB analysis using 23411-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DNAJC21 Polyclonal antibody (23411-1-AP)

    WB analysis of mouse brain using 23411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAJC21 Polyclonal antibody (23411-1-AP)

    WB analysis of HeLa using 23411-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using DNAJC21 Polyclonal antibody (23411-1-AP)

    WB analysis of rat brain using 23411-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DNAJC21 Polyclonal antibody (23411-1-AP)

    IP experiment of HEK-293 using 23411-1-AP

    IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using DNAJC21 Polyclonal antibody (23411-1-AP)

    IHC staining of human pancreas using 23411-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using DNAJC21 Polyclonal antibody (23411-1-AP)

    IHC staining of human pancreas using 23411-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC21 Polyclonal antibody (23411-1-AP)

    IF Staining of HeLa using 23411-1-AP

    Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23411-1-AP targets DNAJC21 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC21 fusion protein Ag20003
Full Name DnaJ (Hsp40) homolog, subfamily C, member 21
Calculated Molecular Weight 531 aa, 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC107577
Gene Symbol DNAJC21
Gene ID (NCBI) 134218
RRIDAB_2879274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

Protocols

Product Specific Protocols
WB protocol for DNAJC21 antibody 23411-1-APDownload protocol
IHC protocol for DNAJC21 antibody 23411-1-APDownload protocol
IF protocol for DNAJC21 antibody 23411-1-APDownload protocol
IP protocol for DNAJC21 antibody 23411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Stem Cell

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Authors - Kaosheng Lv
View Article
humanWB,IF

Am J Hum Genet

DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.

Authors - Hemanth Tummala
  • KD Validated
View Article