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DNAJC3 Polyclonal antibody

DNAJC3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26721-1-AP

Synonyms

DNAJC3, HP58, P58, P58IPK, PRKRI, Protein kinase inhibitor p58

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC3 Polyclonal antibody (26721-1-AP)

    WB analysis using 26721-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26721-1-AP (DNAJC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of human colon cancer using 26721-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of human colon cancer using 26721-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of mouse testis using 26721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJC3 Polyclonal antibody (26721-1-AP)

    IHC staining of mouse testis using 26721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using DNAJC3 Polyclonal antibody (26721-1-AP)

    IF Staining of U2OS using 26721-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 26721-1-AP (DNAJC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26721-1-AP is DNAJC3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC3 fusion protein Ag24966
Full Name DnaJ (Hsp40) homolog, subfamily C, member 3
Observed Molecular Weight58 kDa
GenBank Accession NumberBC047936
Gene Symbol DNAJC3
Gene ID (NCBI) 5611
RRIDAB_2880613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).

Protocols

Product Specific Protocols
WB protocol for DNAJC3 antibody 26721-1-APDownload protocol
IHC protocol for DNAJC3 antibody 26721-1-APDownload protocol
IF protocol for DNAJC3 antibody 26721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

UFMylation of HRD1 regulates endoplasmic reticulum homeostasis

Authors - Hui Luo
View Article
humanWB

FEBS Open Bio

A systems biological analysis of the ATF4-GADD34-CHOP regulatory triangle upon endoplasmic reticulum stress

Authors - Margita Márton
View Article