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DNASE2B Polyclonal antibody

DNASE2B Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22652-1-AP

Synonyms

Deoxyribonuclease 2 beta, deoxyribonuclease II beta, DLAD, DNase II beta, DNase II like acid DNase, DNase2 like acid DNase, DNASE2B, Endonuclease DLAD

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of Jurkat using 22652-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of A549 using 22652-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    IF Staining of A549 using 22652-1-AP

    Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inJurkat cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22652-1-AP is DNASE2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE2B fusion protein Ag18246
Full Name deoxyribonuclease II beta
Calculated Molecular Weight 361 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC126136
Gene Symbol DNASE2B
Gene ID (NCBI) 58511
RRIDAB_2879144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNASE2B antibody 22652-1-APDownload protocol
IHC protocol for DNASE2B antibody 22652-1-APDownload protocol
IF protocol for DNASE2B antibody 22652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Biochem Cell Biol

Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.

Authors - Xiukun Cui
View Article