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DOCK7 Monoclonal antibody

DOCK7 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1A4A6

Cat no : 67842-1-Ig

Synonyms

dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOCK7 Monoclonal antibody (67842-1-Ig)

    WB analysis using 67842-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67842-1-Ig (DOCK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DOCK7 Monoclonal antibody (67842-1-Ig)

    WB analysis using 67842-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67842-1-Ig (DOCK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, PC-12 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, NIH3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67842-1-Ig targets DOCK7 in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DOCK7 fusion protein Ag28417
Full Name dedicator of cytokinesis 7
Calculated Molecular Weight 2109 aa, 239 kDa
Observed Molecular Weight243 kDa
GenBank Accession NumberBC016392
Gene Symbol DOCK7
Gene ID (NCBI) 85440
RRIDAB_2918604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for DOCK7 antibody 67842-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols