EBI3 Monoclonal antibody

EBI3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3H10

Cat no : 66354-1-Ig

Synonyms

EBI3, EBV induced gene 3 protein, Epstein Barr virus induced 3, IL 27 subunit beta, IL 27B, IL27B, Interleukin 27 subunit beta



Tested Applications

Positive WB detected inhuman spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66354-1-Ig targets EBI3 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EBI3 fusion protein Ag3051
Full Name Epstein-Barr virus induced 3
Calculated Molecular Weight 229 aa, 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC015364
Gene Symbol EBI3
Gene ID (NCBI) 10148
RRIDAB_2881734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

Protocols

Product Specific Protocols
WB protocol for EBI3 antibody 66354-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols