EIF3A Monoclonal antibody
EIF3A Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
Human and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C7B4
Cat no : 67713-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, K-562 cells, HeLa cell |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
67713-1-Ig targets EIF3A in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | EIF3A fusion protein Ag24762 |
| Full Name | eukaryotic translation initiation factor 3, subunit A |
| Calculated Molecular Weight | 1382 aa, 166 kDa |
| Observed Molecular Weight | 166 kDa |
| GenBank Accession Number | BC114429 |
| Gene Symbol | EIF3A |
| Gene ID (NCBI) | 8661 |
| RRID | AB_2882903 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| IHC protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| IF protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery | ||
Cell Prolif G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration | ||
Cell Death Differ Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression |
