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  • Featured Product
  • KD/KO Validated

EPB41 Polyclonal antibody

EPB41 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13014-1-AP

Synonyms

4.1R, Band 4.1, E41P, EL1, EPB4.1, EPB41, HE, P4.1, Protein 4.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPB41 Polyclonal antibody (13014-1-AP)

    WB analysis using 13014-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13014-1-AP (EPB41 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using EPB41 Polyclonal antibody (13014-1-AP)

    IP experiment of HL-60 using 13014-1-AP

    IP result of anti-EPB41 (IP:13014-1-AP, 4ug; Detection:13014-1-AP 1:200) with HL-60 cells lysate 4000ug.

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells
Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13014-1-AP targets EPB41 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPB41 fusion protein Ag4075
Full Name erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
Calculated Molecular Weight 720 aa, 81 kDa
Observed Molecular Weight 80 kDa, 135 kDa
GenBank Accession NumberBC039079
Gene Symbol EPB41
Gene ID (NCBI) 2035
RRIDAB_2098362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EPB41 antibody 13014-1-APDownload protocol
IP protocol for EPB41 antibody 13014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Cortical Dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.

Authors - Kiyomitsu Tomomi T
  • KD Validated
View Article
humanWB

EMBO J

NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.

Authors - Sachin Kotak
View Article
humanWB

Front Oncol

Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.

Authors - Shengye Liu
View Article
human,mouseIP

RNA

7SL RNA in Vertebrate Red Blood Cells.

Authors - Gaëlle J S Talhouarne
View Article
WB,IF

Int Immunopharmacol

Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice

Authors - Si-Yao Sang
View Article
mouseWB

Immunology

Cytoskeleton Protein 4.1R Regulates B cell fate by Modulating the Canonical NF-κB Pathway.

Authors - Taotao Liang
  • KO Validated
View Article