Featured Product
KD/KO Validated

TXNDC4 Polyclonal antibody

Name: TXNDC4 Polyclonal antibody
Brand: Proteintech
SKU: 16016-1-AP

TXNDC4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16016-1-AP

Synonyms

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis of HepG2 using 16016-1-AP
WB result of TXNDC4 antibody (16016-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC4 transfected HepG2 cells.
WB analysis using 16016-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16016-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16016-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16016-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16016-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 16016-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16016-1-AP (TXNDC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 16016-1-AP
IP result of anti-TXNDC4 (IP:16016-1-AP, 4ug; Detection:16016-1-AP 1:500) with K-562 cells lysate 4000ug.
IHC staining of human breast cancer using 16016-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16016-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16016-1-AP (TXNDC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16016-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 16016-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16016-1-AP (TXNDC4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 16016-1-AP
Immunofluorescent analysis of Hela cells, using ERP44 antibody 16016-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

-/ -

Cat No. 16016-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Proteintech Guarantee

Request bulk or custom quote

Chat with a team member

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

16016-1-AP targets TXNDC4 in WB, IP, IHC, IF, CoIP applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TXNDC4 fusion protein Ag8818
Full Name	endoplasmic reticulum protein 44
Calculated Molecular Weight	406 aa, 47 kDa
Observed Molecular Weight	44-47 kDa
GenBank Accession Number	BC005374
Gene Symbol	ERP44
Gene ID (NCBI)	23071
RRID	AB_1640025
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for TXNDC4 antibody 16016-1-AP	Download protocol
IHC protocol for TXNDC4 antibody 16016-1-AP	Download protocol
IF protocol for TXNDC4 antibody 16016-1-AP	Download protocol
IP protocol for TXNDC4 antibody 16016-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. Authors - Ke Wang View Article
human	WB,IF	Antioxid Redox Signal Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM). Authors - Anelli Tiziana T View Article
human	WB,IHC,CoIP	J Transl Med ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase. Authors - Hui Tian KD Validated View Article
human	WB	Traffic Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells. Authors - Tiziana Anelli View Article
human,mouse	WB,IHC	BMC Cancer Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma Authors - Tian Xia View Article

TXNDC4 Polyclonal antibody

TXNDC4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Validation Data Gallery

WB analysis of HepG2 using 16016-1-AP

WB analysis using 16016-1-AP

WB analysis of K-562 using 16016-1-AP

WB analysis of HepG2 using 16016-1-AP

WB analysis of HeLa using 16016-1-AP

WB analysis of MCF-7 using 16016-1-AP

WB analysis using 16016-1-AP

IP experiment of K-562 using 16016-1-AP

IHC staining of human breast cancer using 16016-1-AP

IHC staining of human breast cancer using 16016-1-AP

IHC staining of human breast cancer using 16016-1-AP

IHC staining of human breast cancer using 16016-1-AP

IF Staining of HeLa using 16016-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.

Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM).

ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.

Profound architectural and functional readjustments of the secretory pathway in decidualization of endometrial stromal cells.

Exosomal ERp44 derived from ER-stressed cells strengthens cisplatin resistance of nasopharyngeal carcinoma