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FAAH Monoclonal antibody

FAAH Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, rabbit, pig, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C5B5

Cat no : 67607-1-Ig

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis using 67607-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of JAR using 67607-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of LNCaP using 67607-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    IF Staining of LNCaP using 67607-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, JAR cells, LNCaP cells, rabbit brain tissue, pig liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67607-1-Ig targets FAAH in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, rabbit, pig, mouse samples.

Tested Reactivity Human, rat, rabbit, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAAH fusion protein Ag12720
Full Name fatty acid amide hydrolase
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC093632
Gene Symbol FAAH
Gene ID (NCBI) 2166
RRIDAB_2882813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Protocols

Product Specific Protocols
WB protocol for FAAH antibody 67607-1-IgDownload protocol
IHC protocol for FAAH antibody 67607-1-IgDownload protocol
IF protocol for FAAH antibody 67607-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols