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FADS2 Polyclonal antibody
FADS2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28034-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
28034-1-AP targets FADS2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FADS2 fusion protein Ag27709 |
| Full Name | fatty acid desaturase 2 |
| Calculated Molecular Weight | 445 aa, 49 kDa |
| Observed Molecular Weight | 46-52 kDa |
| GenBank Accession Number | BC009011 |
| Gene Symbol | FADS2 |
| Gene ID (NCBI) | 9415 |
| RRID | AB_2918142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FADS2 antibody 28034-1-AP | Download protocol |
| IHC protocol for FADS2 antibody 28034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Chem Biol FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication.
| ||
Int J Mol Sci The Effects of Early-Life Stress on Liver Transcriptomics and the Protective Role of EPA in a Mouse Model of Early-Life-Stress-Induced Adolescent Depression | ||
Int Immunopharmacol E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xianfeng (Verified Customer) (02-18-2020) | Works well with overexpressed mouse FADS2 but no signal with endogenouse mouse FADS2.
 |
FH XIANFENG (Verified Customer) (02-06-2020) | Works well with overexpressed mouse FADS2, but does not work with endogenous mouse FADS2 (mouse liver) .
 |
