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FAM101A Polyclonal antibody

FAM101A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21113-1-AP

Synonyms

FAM101A, Protein FAM101A

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FAM101A Polyclonal antibody (21113-1-AP)

    WB analysis of HepG2 using 21113-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using FAM101A Polyclonal antibody (21113-1-AP)

    WB analysis of human skeletal muscle using 21113-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using FAM101A Polyclonal antibody (21113-1-AP)

    WB analysis of mouse spleen using 21113-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21113-1-AP (FAM101A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FAM101A Polyclonal antibody (21113-1-AP)

    IHC staining of human stomach using 21113-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using FAM101A Polyclonal antibody (21113-1-AP)

    IHC staining of human stomach using 21113-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using FAM101A Polyclonal antibody (21113-1-AP)

    IHC staining of human hepatocirrhosis using 21113-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using FAM101A Polyclonal antibody (21113-1-AP)

    IHC staining of human hepatocirrhosis using 21113-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21113-1-AP (FAM101A antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, human skeletal muscle tissue, mouse spleen tissue
Positive IHC detected inhuman stomach tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21113-1-AP is FAM101A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM101A fusion protein Ag15314
Full Name family with sequence similarity 101, member A
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC141805
Gene Symbol FAM101A
Gene ID (NCBI) 144347
RRIDAB_10733877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM101A antibody 21113-1-APDownload protocol
IHC protocol for FAM101A antibody 21113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols