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FAM124B Polyclonal antibody

FAM124B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21313-1-AP

Synonyms

FAM124B, Protein FAM124B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using FAM124B Polyclonal antibody (21313-1-AP)

    WB analysis of K-562 using 21313-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21313-1-AP (FAM124B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM124B Polyclonal antibody (21313-1-AP)

    IHC staining of human colon cancer using 21313-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21313-1-AP (FAM124B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM124B Polyclonal antibody (21313-1-AP)

    IHC staining of human colon cancer using 21313-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21313-1-AP (FAM124B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21313-1-AP is FAM124B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM124B fusion protein Ag15963
Full Name family with sequence similarity 124B
Calculated Molecular Weight 455 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC025754
Gene Symbol FAM124B
Gene ID (NCBI) 79843
RRIDAB_10859248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM124B is identified as a novel component of CHD7 and CHD8 containing complex. It is a mainly nuclear localized protein with a widespread expressed. Fam124B is a novel protein possibly involved in the pathogenesis of CHARGE syndrome and neurodevelopmental disorders.

Protocols

Product Specific Protocols
WB protocol for FAM124B antibody 21313-1-APDownload protocol
IHC protocol for FAM124B antibody 21313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IHC,IF

PLoS One

Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex.

Authors - Batsukh Tserendulam T
View Article
humanWB

Oncol Lett

New prognostic factors and scoring system for patients with acute myeloid leukemia.

Authors - Ye Kuang
View Article