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  • Featured Product
  • KD/KO Validated

FAM49B Polyclonal antibody

FAM49B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20127-1-AP

Synonyms

BM 009, FAM49B, L1, Protein FAM49B

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FAM49B Polyclonal antibody (20127-1-AP)

    WB analysis of MCF-7 using 20127-1-AP

    WB result of FAM49B antibody (20127-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM49B transfected MCF-7 cells.

  • Western Blot (WB) analysis of mouse brain tissue using FAM49B Polyclonal antibody (20127-1-AP)

    WB analysis of mouse brain using 20127-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FAM49B Polyclonal antibody (20127-1-AP)

    WB analysis of human placenta using 20127-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using FAM49B Polyclonal antibody (20127-1-AP)

    WB analysis of human testis using 20127-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using FAM49B Polyclonal antibody (20127-1-AP)

    WB analysis of mouse testis using 20127-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IP experiment of mouse brain using 20127-1-AP

    IP result of anti-FAM49B (IP:20127-1-AP, 4ug; Detection:20127-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human kidney using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human kidney using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human brain using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human brain using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human pancreas using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FAM49B Polyclonal antibody (20127-1-AP)

    IHC staining of human pancreas using 20127-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM49B Polyclonal antibody (20127-1-AP)

    IF Staining of HepG2 using 20127-1-AP

    Immunofluorescent analysis of HepG2 cells, using FAM49B antibody 20127-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inMCF-7 cells, mouse brain tissue, human placenta tissue, human testis tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20127-1-AP is FAM49B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM49B fusion protein Ag13939
Full Name family with sequence similarity 49, member B
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC003599
Gene Symbol FAM49B
Gene ID (NCBI) 51571
RRIDAB_10638431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAM49B antibody 20127-1-APDownload protocol
IHC protocol for FAM49B antibody 20127-1-APDownload protocol
IF protocol for FAM49B antibody 20127-1-APDownload protocol
IP protocol for FAM49B antibody 20127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

Nat Cell Biol

Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.

Authors - Loic Fort
  • KD Validated
View Article
humanWB

Genes (Basel)

Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.

Authors - Yijian Chen
  • KO Validated
View Article
mouseWB

Mol Immunol

FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.

Authors - Zuotian Huang
  • KD Validated
View Article