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FAM76A Polyclonal antibody

FAM76A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20863-1-AP

Synonyms

FAM76A, Protein FAM76A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM76A Polyclonal antibody (20863-1-AP)

    WB analysis using 20863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20863-1-AP (FAM76A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAM76A Polyclonal antibody (20863-1-AP)

    WB analysis of HeLa using 20863-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20863-1-AP (FAM76A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM76A Polyclonal antibody (20863-1-AP)

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM76A Polyclonal antibody (20863-1-AP)

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20863-1-AP targets FAM76A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM76A fusion protein Ag14904
Full Name family with sequence similarity 76, member A
Calculated Molecular Weight 307 aa, 35 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC025768
Gene Symbol FAM76A
Gene ID (NCBI) 199870
RRIDAB_10693681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM76A is a member of FAM76 family. It is reported that FAM76A may be regulated by WTAP. FAM76A is highly expressed in the WTAP high-expression group and also expressed at low levels in the WTAP low-expression group, which can be confirmed by TCGA data (PMID: 32998774).

Protocols

Product Specific Protocols
WB protocol for FAM76A antibody 20863-1-APDownload protocol
IHC protocol for FAM76A antibody 20863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols