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FBXL16 Polyclonal antibody

FBXL16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25950-1-AP

Synonyms

C16orf22, c380A1.1, F box/LRR repeat protein 16, Fbl16, FBXL16

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FBXL16 Polyclonal antibody (25950-1-AP)

    WB analysis of HeLa using 25950-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25950-1-AP (FBXL16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using FBXL16 Polyclonal antibody (25950-1-AP)

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using FBXL16 Polyclonal antibody (25950-1-AP)

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 25950-1-AP is FBXL16 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL16 fusion protein Ag22827
Full Name F-box and leucine-rich repeat protein 16
Calculated Molecular Weight 479 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC036680
Gene Symbol FBXL16
Gene ID (NCBI) 146330
RRIDAB_2880307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FBXL16 antibody 25950-1-APDownload protocol
IHC protocol for FBXL16 antibody 25950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Chem Biol

LATS2 condensates organize signalosomes for Hippo pathway signal transduction

Authors - Min Qin
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Nazia (Verified Customer) (01-06-2022)

Package arrived very quickly and staff were very helpful. Product worked just as expected. Great customer service. I will be recommending ProteinTech to colleagues in my lab.

  • Applications: Western Blot