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FGF18 Monoclonal antibody

FGF18 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D11D12

Cat no : 60341-1-Ig

Synonyms

FGF 18, FGF18, fibroblast growth factor 18, ZFGF5

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using FGF18 Monoclonal antibody (60341-1-Ig)

    WB analysis of HT-1080 using 60341-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 60341-1-Ig (FGF18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FGF18 Monoclonal antibody (60341-1-Ig)

    WB analysis of NIH/3T3 using 60341-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60341-1-Ig (FGF18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHT-1080 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60341-1-Ig targets FGF18 in WB, ELISA applications and shows reactivity with human, mouse, rabbit samples.

Tested Reactivity human, mouse, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FGF18 fusion protein Ag2041
Full Name fibroblast growth factor 18
Calculated Molecular Weight 207 aa, 24 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC006245
Gene Symbol FGF18
Gene ID (NCBI) 8817
RRIDAB_2881450
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Protocols

Product Specific Protocols
WB protocol for FGF18 antibody 60341-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.

Authors - Kai Guo
View Article