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FHL5 Polyclonal antibody

FHL5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12650-2-AP

Synonyms

ACT, Activator of CREM in testis, FHL 5, FHL5, four and a half LIM domains 5, RP3 393D12.2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using FHL5 Polyclonal antibody (12650-2-AP)

    WB analysis of mouse heart using 12650-2-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12650-2-AP (FHL5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using FHL5 Polyclonal antibody (12650-2-AP)

    WB analysis of human heart using 12650-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12650-2-AP (FHL5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FHL5 Polyclonal antibody (12650-2-AP)

    WB analysis of mouse heart using 12650-2-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12650-2-AP (FHL5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FHL5 Polyclonal antibody (12650-2-AP)

    WB analysis of mouse heart using 12650-2-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12650-2-AP (FHL5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12650-2-AP targets FHL5 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FHL5 fusion protein Ag3364
Full Name four and a half LIM domains 5
Calculated Molecular Weight 284 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC021723
Gene Symbol FHL5
Gene ID (NCBI) 9457
RRIDAB_2104496
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FHL5 antibody 12650-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols