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FJX1 Polyclonal antibody

FJX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17417-1-AP

Synonyms

FJX1, Four jointed box protein 1, Four jointed protein homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    WB analysis of rat brain using 17417-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17417-1-AP (FJX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse brain using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse brain using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of human liver using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of human liver using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse testis using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FJX1 Polyclonal antibody (17417-1-AP)

    IHC staining of mouse testis using 17417-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue, human liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17417-1-AP targets FJX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FJX1 fusion protein Ag11385
Full Name four jointed box 1 (Drosophila)
Calculated Molecular Weight 437 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC049171
Gene Symbol FJX1
Gene ID (NCBI) 24147
RRIDAB_2878406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.

Protocols

Product Specific Protocols
WB protocol for FJX1 antibody 17417-1-APDownload protocol
IHC protocol for FJX1 antibody 17417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Cell Endocrinol

Regulation of Sertoli cell function by planar cell polarity (PCP) protein Fjx1

Authors - Tiao Bu
View Article