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  • Featured Product
  • KD/KO Validated

FKBP8 Polyclonal antibody

FKBP8 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11173-1-AP

Synonyms

38 kDa FK506 binding protein, 38 kDa FKBP, FK506 binding protein 8, FK506 binding protein 8, 38kDa, FKBP 38, FKBP 8, FKBP38, FKBP8, FKBPr38, hFKBP38, PPIase FKBP8, Rotamase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of HeLa using 11173-1-AP

    WB result of FKBP8 antibody (11173-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis using 11173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of mouse brain using 11173-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of HeLa using 11173-1-AP

    WB result of FKBP8 antibody (11173-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP8 transfected HeLa cells.

  • Western Blot (WB) analysis of Jurkat cells using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of Jurkat using 11173-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of HeLa using 11173-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of mouse liver using 11173-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of SH-SY5Y using 11173-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of mouse brain using 11173-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using FKBP8 Polyclonal antibody (11173-1-AP)

    WB analysis of mouse pancreas using 11173-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11173-1-AP (FKBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using FKBP8 Polyclonal antibody (11173-1-AP)

    IP experiment of HeLa using 11173-1-AP

    IP result of anti-FKBP8 (IP:11173-1-AP, 4ug; Detection:11173-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using FKBP8 Polyclonal antibody (11173-1-AP)

    IF Staining of SH-SY5Y using 11173-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 11173-1-AP (FKBP8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse pancreas tissue, Jurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11173-1-AP targets FKBP8 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP8 fusion protein Ag1649
Full Name FK506 binding protein 8, 38kDa
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_10597097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FKBP8 antibody 11173-1-APDownload protocol
IF protocol for FKBP8 antibody 11173-1-APDownload protocol
IP protocol for FKBP8 antibody 11173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali
View Article
human

Front Bioeng Biotechnol

Efficient and reversible Cas13d-mediated knockdown with an all-in-one lentivirus-vector

Authors - Suli Lv
  • KO Validated
View Article
ratIF

Int J Mol Sci

Analysis of Dysferlin Direct Interactions with Putative Repair Proteins Links Apoptotic Signaling to Ca2+ Elevation via PDCD6 and FKBP8

Authors - Dennis G Drescher
View Article
humanWB

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
  • KO Validated
View Article
humanWB

Mol Hum Reprod

Comprehensive analysis of ovarian granulosa cell proteomics and phosphoproteomics in PCOS patients without insulin resistance

Authors - Xiao Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells