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FSD1L Polyclonal antibody

FSD1L Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21032-1-AP

Synonyms

CCDC10, CSDUFD1, FSD1 like protein, FSD1 N terminal like protein, FSD1CL, FSD1L, FSD1NL, MIR1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using FSD1L Polyclonal antibody (21032-1-AP)

    IHC staining of human brain using 21032-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21032-1-AP (FSD1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FSD1L Polyclonal antibody (21032-1-AP)

    IHC staining of human brain using 21032-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21032-1-AP (FSD1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FSD1L Polyclonal antibody (21032-1-AP)

    IF Staining of HepG2 using 21032-1-AP

    Immunofluorescent analysis of HepG2 cells, using FSD1L antibody 21032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FSD1L Polyclonal antibody (21032-1-AP)

    IF Staining of HeLa using 21032-1-AP

    Immunofluorescent analysis of Hela cells, using FSD1L antibody 21032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using FSD1L Polyclonal antibody (21032-1-AP)

    IF Staining of HEK-293 using 21032-1-AP

    Immunofluorescent analysis of 293 cells, using FSD1L antibody 21032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells, Hela cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21032-1-AP targets FSD1L in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSD1L fusion protein Ag15345
Full Name fibronectin type III and SPRY domain containing 1-like
Calculated Molecular Weight 530 aa, 60 kDa
Observed Molecular Weight 56-65 kDa
GenBank Accession NumberBC036746
Gene Symbol FSD1L
Gene ID (NCBI) 83856
RRIDAB_10732808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for FSD1L antibody 21032-1-APDownload protocol
IF protocol for FSD1L antibody 21032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols