FUBP1 Polyclonal antibody
FUBP1 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 24864-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, Jurkat cells, K-562 cells, mouse brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| ChIP | See 1 publications below |
Product Information
The immunogen of 24864-1-AP is FUBP1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FUBP1 fusion protein Ag13149 |
| Full Name | far upstream element (FUSE) binding protein 1 |
| Calculated Molecular Weight | 653 aa, 69 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC017247 |
| Gene Symbol | FUBP1 |
| Gene ID (NCBI) | 8880 |
| RRID | AB_2879762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| IF protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| IP protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Poly(U) binding splicing factor 60 promotes renal cell carcinoma growth by transcriptionally upregulating telomerase reverse transcriptase. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Anticancer Res Comparative Proteomic Profiling of Cisplatin-resistant Nasopharyngeal Carcinoma Cell Lines: Novel Biomarkers for Improving Chemotherapy of NPC. | ||
J Adv Res LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1 | ||
MedComm (2020) Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma | ||
J Immunother Cancer The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development
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