FUCA2 Polyclonal antibody
FUCA2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15157-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, mouse stomach tissue, rat stomach tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15157-1-AP targets FUCA2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FUCA2 fusion protein Ag7250 |
| Full Name | fucosidase, alpha-L- 2, plasma |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54-60 kDa |
| GenBank Accession Number | BC003060 |
| Gene Symbol | FUCA2 |
| Gene ID (NCBI) | 2519 |
| RRID | AB_2104956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUCA2 antibody 15157-1-AP | Download protocol |
| IHC protocol for FUCA2 antibody 15157-1-AP | Download protocol |
| IP protocol for FUCA2 antibody 15157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Gretchen (Verified Customer) (02-01-2019) | I did a 1:2000 dilution in 30ug of lysate protein. The bands were sharp and strong and it took 3 seconds to develop. However, I had a lot of unspecific bands and not only at 86, they were some stronger than others.
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