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Frizzled 8 Polyclonal antibody

Frizzled 8 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55093-1-AP

Synonyms

Frizzled 8, FZ 8, FZD8, hFZ8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Frizzled 8 Polyclonal antibody (55093-1-AP)

    WB analysis of HeLa using 55093-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse embryo tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    WB analysis of mouse embryo using 55093-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    WB analysis of mouse lung using 55093-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of multi-cells/tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    WB analysis of multi-cells/tissue using 55093-1-AP

    WB result of 55093-1-AP (Frizzled 8 antibody).

  • Western Blot (WB) analysis of mouse heart tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    WB analysis of mouse heart using 55093-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55093-1-AP (Frizzled 8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    IHC staining of human pancreas cancer using 55093-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Frizzled 8 Polyclonal antibody (55093-1-AP)

    IHC staining of human pancreas cancer using 55093-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55093-1-AP (Frizzled 8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse embryo tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55093-1-AP targets Frizzled 8 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name frizzled homolog 8 (Drosophila)
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-73 kDa
GenBank Accession NumberNM_031866
Gene Symbol FZD8
Gene ID (NCBI) 8325
RRIDAB_2881267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Frizzled 8 antibody 55093-1-APDownload protocol
IHC protocol for Frizzled 8 antibody 55093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu
View Article