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G6PC Monoclonal antibody

G6PC Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E11A5

Cat no : 66860-1-Ig

Synonyms

G 6 Pase, G6P, G6Pase, G6Pase alpha, G6PC, G6PT, Glucose 6 phosphatase, Glucose 6 phosphatase alpha, GSD1, GSD1a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G6PC Monoclonal antibody (66860-1-Ig)

    WB analysis using 66860-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HuH-7 cells using G6PC Monoclonal antibody (66860-1-Ig)

    WB analysis of HuH-7 using 66860-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using G6PC Monoclonal antibody (66860-1-Ig)

    WB analysis of L02 using 66860-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66860-1-Ig (G6PC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using G6PC Monoclonal antibody (66860-1-Ig)

    IHC staining of human liver using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using G6PC Monoclonal antibody (66860-1-Ig)

    IHC staining of human liver using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using G6PC Monoclonal antibody (66860-1-Ig)

    IHC staining of human breast cancer using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using G6PC Monoclonal antibody (66860-1-Ig)

    IHC staining of human breast cancer using 66860-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66860-1-Ig (G6PC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHuH-7 cells, L02 cells, HepG2 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66860-1-Ig targets G6PC in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G6PC fusion protein Ag17839
Full Name glucose-6-phosphatase, catalytic subunit
Calculated Molecular Weight 357 aa, 40 kDa
Observed Molecular Weight 37-42 kDa
GenBank Accession NumberBC130478
Gene Symbol G6PC
Gene ID (NCBI) 2538
RRIDAB_2882199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

Protocols

Product Specific Protocols
WB protocol for G6PC antibody 66860-1-IgDownload protocol
IHC protocol for G6PC antibody 66860-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pharmacol

Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota

Authors - Sa Yang
View Article
humanWB

Front Cell Dev Biol

Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma

Authors - Feng Cao
View Article