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GAL3ST3 Polyclonal antibody

GAL3ST3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24851-1-AP

Synonyms

GAL3ST 3, GAL3ST3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GAL3ST3 Polyclonal antibody (24851-1-AP)

    WB analysis of mouse brain using 24851-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24851-1-AP (GAL3ST3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GAL3ST3 Polyclonal antibody (24851-1-AP)

    WB analysis of mouse kidney using 24851-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24851-1-AP (GAL3ST3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using GAL3ST3 Polyclonal antibody (24851-1-AP)

    IF Staining of mouse heart using 24851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 24851-1-AP (GAL3ST3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using GAL3ST3 Polyclonal antibody (24851-1-AP)

    IF Staining of mouse heart using 24851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 24851-1-AP (GAL3ST3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24851-1-AP targets GAL3ST3 in WB, IF, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAL3ST3 fusion protein Ag20692
Full Name galactose-3-O-sulfotransferase 3
Calculated Molecular Weight 431 aa, 49 kDa
Observed Molecular Weight 49-50 kDa
GenBank Accession NumberBC113552
Gene Symbol GAL3ST3
Gene ID (NCBI) 89792
RRIDAB_2879756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GAL3ST3 antibody 24851-1-APDownload protocol
IF protocol for GAL3ST3 antibody 24851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols