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  • Featured Product
  • KD/KO Validated

GALK1 Polyclonal antibody

GALK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15284-1-AP

Synonyms

Galactokinase, galactokinase 1, Galactose kinase, GALK, GALK1, GK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALK1 Polyclonal antibody (15284-1-AP)

    WB analysis using 15284-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15284-1-AP (GALK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using GALK1 Polyclonal antibody (15284-1-AP)

    WB analysis of mouse heart using 15284-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15284-1-AP (GALK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GALK1 Polyclonal antibody (15284-1-AP)

    IHC staining of human breast cancer using 15284-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15284-1-AP (GALK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GALK1 Polyclonal antibody (15284-1-AP)

    IF Staining of MCF-7 using 15284-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15284-1-AP (GALK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue, Y79 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15284-1-AP targets GALK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALK1 fusion protein Ag7403
Full Name galactokinase 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001166
Gene Symbol GALK1
Gene ID (NCBI) 2584
RRIDAB_2108553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALK1, also named as GALK, belongs to the GHMP kinase family and GalK subfamily. GALK1, located on chromosome 17q24, encodes galactokinase and is involved in galactose metabolism (PMID: 32807972). The molecular weight of GALK1 is 42 kDa.

Protocols

Product Specific Protocols
WB protocol for GALK1 antibody 15284-1-APDownload protocol
IHC protocol for GALK1 antibody 15284-1-APDownload protocol
IF protocol for GALK1 antibody 15284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.

Authors - Gek San Tan
  • KD Validated
View Article
pigWB

Eur J Nutr

Epigenetic and SP1-mediated regulation is involved in the repression of galactokinase 1 gene in the liver of neonatal piglets born to betaine-supplemented sows.

Authors - Demin Cai
View Article