GALNT3 Polyclonal antibody

Name: GALNT3 Polyclonal antibody
Brand: Proteintech
SKU: 16716-1-AP

GALNT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16716-1-AP

Synonyms

GalNAc T3, GALNT3, HFTC, HHS, pp GaNTase 3

Validation Data Gallery

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AllWBIHC

WB analysis of PC-3 using 16716-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16716-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of DU 145 using 16716-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16716-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse liver using 16716-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of Transfected HEK-293 using 16716-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16716-1-AP (GALNT3 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 16716-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16716-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16716-1-AP (GALNT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 16716-1-AP

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Tested Applications

Positive WB detected in	PC-3 cells, DU 145 cells, HeLa cells, mouse liver tissue, mouse testis tissue, Transfected HEK-293 cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 2 publications below

Product Information

16716-1-AP targets GALNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GALNT3 fusion protein Ag10140
Full Name	UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3)
Calculated Molecular Weight	633aa,73 kDa; 192aa,22 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC056246
Gene Symbol	GALNT3
Gene ID (NCBI)	2591
RRID	AB_2878303
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GALNT3 antibody 16716-1-AP	Download protocol
IHC protocol for GALNT3 antibody 16716-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Cycle LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway. Authors - Yue Li View Article
mouse	IHC	Bone Growth-related skeletal changes and alterations in phosphate metabolism. Authors - Toshimi Michigami View Article
human	WB	J Exp Clin Cancer Res LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway. Authors - Bing Liu View Article
human	IHC	Hum Cell GalNAc-T3 and MUC1, a combined predictor of prognosis and recurrence in solitary pulmonary adenocarcinoma initially diagnosed as malignant solitary pulmonary nodule (≤ 3 cm). Authors - Qiang Xie View Article
rat	WB	Exp Ther Med PMID: 29805509 Authors - Yanshao Liu View Article
mouse	WB	Environ Toxicol Cisplatin induces acute kidney injury by downregulating miR-30e-5p that targets Galnt3 to activate the AMPK signaling pathway Authors - Weipu Mao View Article

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Research Area

GALNT3 Polyclonal antibody

GALNT3 Polyclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis of PC-3 using 16716-1-AP

WB analysis of mouse testis using 16716-1-AP

WB analysis of DU 145 using 16716-1-AP

WB analysis of HeLa using 16716-1-AP

WB analysis of mouse liver using 16716-1-AP

WB analysis of Transfected HEK-293 using 16716-1-AP

IHC staining of mouse kidney using 16716-1-AP

IHC staining of mouse kidney using 16716-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.

Growth-related skeletal changes and alterations in phosphate metabolism.

LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway.

GalNAc-T3 and MUC1, a combined predictor of prognosis and recurrence in solitary pulmonary adenocarcinoma initially diagnosed as malignant solitary pulmonary nodule (≤ 3 cm).

PMID: 29805509

Cisplatin induces acute kidney injury by downregulating miR-30e-5p that targets Galnt3 to activate the AMPK signaling pathway