GBA Polyclonal antibody

GBA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27972-1-AP

Synonyms

Acid beta glucosidase, Alglucerase, Beta glucocerebrosidase, GBA, GBA1, GC, GCB, GLUC, Glucosylceramidase, Imiglucerase



Tested Applications

Positive WB detected inSH-SY5Y cells, HeLa cells
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27972-1-AP targets GBA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBA fusion protein Ag27388
Full Name glucosidase, beta; acid (includes glucosylceramidase)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC003356
Gene Symbol GBA
Gene ID (NCBI) 2629
RRIDAB_2881024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GBA antibody 27972-1-APDownload protocol
IHC protocol for GBA antibody 27972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins

Authors - Fatema Akter
mouseWB

Sci Total Environ

Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10

Authors - Zhuo Ma