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  • Featured Product
  • KD/KO Validated

GBF1 Polyclonal antibody

GBF1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25183-1-AP

Synonyms

ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBF1 Polyclonal antibody (25183-1-AP)

    WB analysis of HeLa using 25183-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25183-1-AP (GBF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GBF1 Polyclonal antibody (25183-1-AP)

    IHC staining of human prostate cancer using 25183-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GBF1 Polyclonal antibody (25183-1-AP)

    IHC staining of human prostate cancer using 25183-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25183-1-AP (GBF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBF1 Polyclonal antibody (25183-1-AP)

    IF Staining of HeLa using 25183-1-AP

    Immunofluorescent analysis of HeLa cells using 25183-1-AP (GBF1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25183-1-AP is GBF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBF1 fusion protein Ag16579
Full Name golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1
Calculated Molecular Weight 1859 aa, 206 kDa
Observed Molecular Weight 206 kDa
GenBank Accession NumberBC117681
Gene Symbol GBF1
Gene ID (NCBI) 8729
RRIDAB_2879948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GBF1 antibody 25183-1-APDownload protocol
IHC protocol for GBF1 antibody 25183-1-APDownload protocol
IF protocol for GBF1 antibody 25183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
View Article
humanWB

Sci Rep

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Authors - Xiaobo Lei
  • KD Validated
View Article