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GMAP-210 Polyclonal antibody
GMAP-210 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26456-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HL-60 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
26456-1-AP targets GMAP-210 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GMAP-210 fusion protein Ag13867 |
| Full Name | thyroid hormone receptor interactor 11 |
| Calculated Molecular Weight | 1979 aa, 228 kDa |
| Observed Molecular Weight | 230 kDa |
| GenBank Accession Number | BC002656 |
| Gene Symbol | TRIP11 |
| Gene ID (NCBI) | 9321 |
| RRID | AB_2880519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GMAP-210 antibody 26456-1-AP | Download protocol |
| IHC protocol for GMAP-210 antibody 26456-1-AP | Download protocol |
| IF protocol for GMAP-210 antibody 26456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Tumor protein D54 binds intracellular nanovesicles via an extended amphipathic region. | ||
Commun Biol Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. | ||
Hum Mutat Biallelic deep intronic variant c.5457+81T>A in TRIP11 causes loss of function and results in achondrogenesis 1A. | ||
Biol Open GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (04-28-2023) | clean band around the expected size
|
FH Yuki (Verified Customer) (01-24-2023) | I will recommend this antibody for IF
 |
